AB 11:127-138 (2010)  -  DOI: https://doi.org/10.3354/ab00298

Microalgal pigment ratios in relation to light intensity: implications for chemotaxonomy

C. S. Grant, J. W. Louda*

Organic Geochemistry Group, Department of Chemistry and Biochemistry, and The Environmental Sciences Program, Florida Atlantic University, 777 Glades Road, Boca Raton, Florida 33431, USA
*Corresponding author. Email:

ABSTRACT: Pigment-based chemotaxonomy relies upon the assertion that the pigment arrays of mixed microalgal communities (phytoplankton, periphyton, microphytobenthos) can be deconvoluted in ways to provide an estimate of taxon-specific chlorophyll a (chl a) as a proxy for biomass. This relies on having valid chl a:biomarker pigment ratios. However, pigment ratios change for a variety of reasons. We examined the effects of only light intensity (photon flux density, PFD) on the pigment ratios of 10 species in the phyla Cyanobacteria, Chlorophyta, Chromophyta (Bacillariophyceae), Haptophyta, and Pyrrophyta. Chl a:marker pigment versus PFD (30–45 through 1800 µmol quanta m–2 s–1) data revealed distinct trends for photosynthetic accessory pigments (PAP) versus photoprotective pigments (PPP). Chl a:PAP class pigments (chl b, fucoxanthin, peridinin, echinenone) exhibited relative stability (possibly stoichiometry) with increasing PFD, while chl a:PPP class pigment trends revealed large increases in the relative amounts of the PPP pigments (zeaxanthin, myxoxanthophyll, canthaxanthin, scytonemins). Total chl a cell–1 often decreases in concert with increasing PFD and partially or totally explains the decrease in chl a:PPP ratios. We verified that all ‘xanthophyll cycle’ pigments expressed large and erratic ratios and therefore did not predict chl a levels. We conclude that light (400 to 700 nm) measurements taken in the field can offer valuable ancillary data for adjusting pigment-based chemotaxonomic formulae for the study of microalgal communities.


KEY WORDS: Chemotaxonomy · Light · Pigments · Biomarker ratios · Chlorophylls · Carotenoids · Photoprotection


Full text in pdf format
Erratum
Supplementary material
 
Cite this article as: Grant CS, Louda JW (2010) Microalgal pigment ratios in relation to light intensity: implications for chemotaxonomy. Aquat Biol 11:127-138. https://doi.org/10.3354/ab00298

Export citation
Mail this link - Contents Mailing Lists - RSS
- -