AB 11:157-162 (2010)  -  DOI: https://doi.org/10.3354/ab00303

High-hydrostatic-pressure optical chamber system for cultivation and microscopic observation of deep-sea organisms

Chengman Bao1, Yun Gai2, Kaikai Lou2, Chunyue Jiang3, Shuming Ye2,*

1College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China
2College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou 310027, PR China
3College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou 310014, PR China
*Corresponding author. Email:

ABSTRACT: We developed and tested a high-pressure optical chamber system for cultivation and microscopic observation of deep-sea organisms. The system is composed of an optical chamber, a high-pressure pump, a pressure sensor and a microscope. The chamber has an observation cavity, 2 cultivation cavities and 2 sapphire windows. The pump is employed for perfusion of culture medium and for increasing the pressure. The pressure sensor monitors the pressure in the chamber. The microscope is used for observing samples through the sapphire windows. In the future, the optical chamber system could be used alone for short-term research or be connected to a large high-pressure vessel to create a flow-through system for long-term research. Using the system, the swimming activity of Bosmina longirostris (Branchiopoda: Cladocera) was observed at different pressures. Swimming activity increased with increasing compression up to 30 MPa. During decompression, this activity reappeared when pressure decreased to 45 MPa and increased with further decreasing pressure.


KEY WORDS: Optical chamber system · Real-time · Microscopic observation · Plankton


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Cite this article as: Bao C, Gai Y, Lou K, Jiang C, Ye S (2010) High-hydrostatic-pressure optical chamber system for cultivation and microscopic observation of deep-sea organisms. Aquat Biol 11:157-162. https://doi.org/10.3354/ab00303

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