AB 13:243-250 (2011)  -  DOI: https://doi.org/10.3354/ab00365

Characterization and evaluation of cholinesterase activity in the cockle Cerastoderma glaucum

Jamel Jebali*, Sana Ben-Khedher, Nawel Kamel, Jihene Ghedira, Zied Bouraoui, Hamadi Boussetta

Laboratory of Biochemical and Environmental Toxicology, Higher Institute of Agriculture, Chott-Mariem, 4042-Sousse, Tunisia

ABSTRACT: The aim of the present study was to partially characterize the biochemical properties of cholinesterase (ChE) activity in the tissues of the cockle Cerastoderma glaucum, using different substrates and sample volumes, and to evaluate ChE response following caging exposure in a fishing harbour. Comparative analysis of the Michaelis-Menten kinetics in the studied tissues of C. glaucum indicated that ChE activity, using acetylthiocholine as substrate, displayed a tissue-dependent response, with activity levels in the following order of magnitude: whole animal > rest of animal (adductor and retractor muscles, mantle and foot) > visceral mass > gills. The transfer of C. glaucum to the Téboulba fishing harbour caused a time-dependent reduction in acetylcholinesterase activity.


KEY WORDS: Biomarker · Cockle · Cerastoderma glaucum · Cholinesterase · ChE · Caging · Fishing harbour


Full text in pdf format 
Cite this article as: Jebali J, Ben-Khedher S, Kamel N, Ghedira J, Bouraoui Z, Boussetta H (2011) Characterization and evaluation of cholinesterase activity in the cockle Cerastoderma glaucum. Aquat Biol 13:243-250. https://doi.org/10.3354/ab00365

Export citation
Mail this link - Contents Mailing Lists - RSS
- -