AB 26:69-73 (2017)  -  DOI: https://doi.org/10.3354/ab00675

Assignment of parentage by microsatellite analysis in the endangered Brachymystax lenok tsinlingensis (Salmonidae)

Jian Shao1,2,3, Wei Luo4, Qiwei Wei1,2,*, Feng Wang5, Wei Guo2, Huan Ye2, Zhipeng Chu2, Jinming Wu2, Shuhuan Zhang

1College of Fisheries, Huazhong Agricultural University, Wuhan 430070, PR China
2Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, PR China
3College of Animal Science, Guizhou University, GuiYang 550025, PR China
4South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China
5Yellow River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Xi’an 710086, PR China
*Corresponding author:

ABSTRACT: We evaluated the use of microsatellite markers to identify parentage in Brachymystax lenok tsinlingensis Li, 1966, an endangered salmonid. Computer simulations showed that when no parent information was available, or when information was only available from 1 parent, then exclusion probabilities ranged from 23.6 to 45.1% and from 25.9 to 52.4%, respectively; combined exclusion probabilities for the 8 loci investigated were 97.7 and 98.4%, respectively. However, a breeding experiment with known parental and filial information resulted in 91.28% of progeny being exclusively assigned to their correct parent pair. Our data demonstrate that microsatellite-based parentage assignment is a reliable means with which to obtain information pertaining to genealogical relationships and could therefore benefit genetic conservation of this species.


KEY WORDS: Brachymystax lenok tsinlingensis · Microsatellites · Parentage assignment · Inbreeding depression


Full text in pdf format  
Cite this article as: Shao J, Luo W, Wei Q, Wang F and others (2017) Assignment of parentage by microsatellite analysis in the endangered Brachymystax lenok tsinlingensis (Salmonidae). Aquat Biol 26:69-73. https://doi.org/10.3354/ab00675

Export citation
Mail this link - Contents Mailing Lists - RSS
- -