AB 3:283-290 (2008)  -  DOI: https://doi.org/10.3354/ab00089

Activity of Na+,K+-ATPase in a ‘freshwater shrimp’, Palaemonetes argentinus (Caridea, Palaemonidae): ontogenetic and salinity-induced changes

Romina B. Ituarte1,4,*, Alejandra A. López Mañanes2,4, Eduardo D. Spivak1,4, Klaus Anger3

1Laboratorio Zoología Invertebrados, Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Casilla de Correo 1245, 7600 Mar del Plata, Argentina
2Laboratorio Fisiología Bioquímica y Adaptativa, Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Funes 3250, Mar del Plata B 7602 AYJ, Argentina
3Biologische Anstalt Helgoland, Stiftung Alfred-Wegener-Institut für Polar- und Meeresforschung, Meeresstation,
27498 Helgoland, Germany
4Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina

ABSTRACT: Embryos, larvae, and adults of Palaemonetes argentinus tolerate a wide range of salinities (1 to 25‰). While osmoregulatory capacities have previously been demonstrated in all post-embryonic stages, little is known about the occurrence of osmoregulation during the embryonic phase. We examined ontogenetic and salinity-induced changes in the activity of a key enzyme involved in osmoregulation, Na+,K+-ATPase. Its activity was studied in: (1) eggs at an early (SI), an intermediate (SII), and a late stage of embryonic development (SIII); (2) in newly hatched larvae (Zoea-I, ZI); and in homogenates of (3) whole adults and (4) isolated gill tissue. All stages were directly exposed to 1, 15, or 25‰, and Na+,K+-ATPase activity was chemically determined 24 h (embryos, larvae) or 48 h later (adults). Enzyme activity was detected in all developmental stages, being low in SI and SII, maximum in SIII, and intermediate in ZI and adults. Maximum salinity-induced activity changes prior to hatching (SIII) suggest that hyper-osmoregulatory functions are expressed by the end of the embryonic phase. The ontogenetic activity maximum at this stage, however, may also be related to the hatching process. Comparing different salinities, Na+,K+-ATPase activity in SIII was always highest at 15‰, whereas the activity in gills was higher at both 15 and 25‰ than at 1‰. While gills are absent in the embryonic and early larval stages, ion-transporting cells must be located elsewhere during these early ontogenetic stages, probably in the brachiostegites.


KEY WORDS: Embryos · Salinity tolerance · Na+,K+-ATPase · Palaemonid shrimp · Gill


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Cite this article as: Ituarte RB, López Mañanes AA, Spivak ED, Anger K (2008) Activity of Na+,K+-ATPase in a ‘freshwater shrimp’, Palaemonetes argentinus (Caridea, Palaemonidae): ontogenetic and salinity-induced changes. Aquat Biol 3:283-290. https://doi.org/10.3354/ab00089

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