AME 11:11-19 (1996)  -  doi:10.3354/ame011011

CO2 production predicted from isocitrate dehydrogenase activity and bisubstrate enzyme kinetics in the marine bacterium Pseudomonas nautica

Packard T, Berdalet E, Blasco D, Roy SO, St-Amand L, Lagacé B, Lee K, Gagné JP

CO2 production in aerobic bacteria was modeled from the time-courses of the in vitro activity of isocitrate dehydrogenase (IDH), bacterial protein, and the concentration of the carbon source in the cultures. The model was based on the concept of bisubstrate control of the IDH reaction throughout the exponential, steady-state, and senescent phases of the cultures. In the exponential phase, the measured rates of CO2 production and the in vitro IDH activity were closely coupled, but in the senescent phase, they became uncoupled. The in vitro IDH activity remained high even after the culture's carbon source was exhausted, while the CO2 production fell to low levels. Based on the hypothesis that this uncoupling was caused by internal substrate limitation, 2 mathematical models incorporating a bisubstrate enzyme kinetics algorithm were constructed and tested. The models predicted the rate of CO2 production throughout the different phases of the cultures with an r2 greater than 0.84.

Respiration · Metabolism · Bacteria · Carbon dioxide · Ocean model · Isocitrate dehydrogenase activity

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