AME 45:229-235 (2006)  -  doi:10.3354/ame045229

Bacterioplankton composition in the Scotia Sea, Antarctica, during the austral summer of 2003

Juliette N. Topping1,*, Jane L. Heywood1, Peter Ward2, Mikhail V. Zubkov1

1National Oceanography Centre, European Way, Southampton SO14 3ZH, UK
2British Antarctic Survey, Natural Environment Research Council, High Cross, Madingley Road, Cambridge CB3 0ET, UK

ABSTRACT: Physical ocean processes (ice-melt, island run-off and upwelling of nutrients) were hypothesised to affect the bacterioplankton composition in the surface mixed layer of the Scotia Sea during the austral summer of 2003, and this was investigated using flow cytometry and catalysed reporter deposition fluorescence in situ hybridisation (CARD-FISH) techniques. The bacterioplankton was composed predominantly of Alphaproteobacteria (PB), comprising SAR11, Roseobacter spp. and SAR116 groups, followed by Sphingobacteria/Flavobacteria and Gammaproteobacteria, including SAR86. Two distinct bacterioplankton communities were identified, largely based on bacterioplankton abundance, which varied from 0.3 ± 0.06 × 106 cells ml–1 in the west to 0.8 ± 0.3 × 106 cells ml–1 in the east, and a corresponding difference in SAR11 percentages of 30 ± 15% in the west compared to 5 ± 5% in the east. The western community was present in waters that were largely in an over-wintered, pre-bloom condition. The eastern bacterioplankton community was associated with phytoplankton blooms developed within the eastern Scotia Sea nutrient upwelling zone, where the Antarctic Circumpolar Current (ACC) encounters the shallow bathymetry associated with the Scotia Arc, in combination with seasonal ice-melt and island effects that enabled surface water stratification.

KEY WORDS: Bacterioplankton · Community · CARD-FISH · Flow Cytometry · Scotia Sea · Antarctic Circumpolar Current

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