AME 45:247-254 (2006) - doi:10.3354/ame045247
Seasonal changes and diversity of aerobic anoxygenic phototrophs in the Baltic Sea
Michal Masín1,2, Agnieszka Zdun3, Joanna Ston-Egiert3, Monika Nausch4, Matthias Labrenz4, Vladimíra Moulisová2, Michal Koblízek1,2,*
ABSTRACT: The community of aerobic anoxygenic phototrophs was investigated in the Baltic Sea using infrared epifluorescence microscopy from September 2004 to October 2005. The majority of these bacteriochlorophyll-containing organisms exhibited a specific sickle-shaped morphology, with rods or other morphotypes observed only occasionally. Aerobic anoxygenic phototrophs were observed mostly from April to September (1 to 12% of total prokaryotes), peaking in May 2005 at a concentration of up to 0.38 × 106 cells ml1. This peak was associated with the later phase of the spring bloom. In the later months, the amount of phototrophic bacteria gradually declined until the beginning of the fall mixing, and remained low from November to March, contributing only 0 to 2% of total prokaryotes. A novel technique combining fluorescent in situ hybridization (FISH) and infrared epifluorescence microscopy indicated that the Baltic aerobic anoxygenic phototrophs were mostly Gammaproteobacteria, with a smaller fraction of Alphaproteobacteria.
KEY WORDS: Aerobic photosynthetic bacteria · Bacteriochlorophyll a · Photoheterotrophy ·Epifluorescence microscopy
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