AME 50:113-122 (2008)  -  DOI: https://doi.org/10.3354/ame01157

Clone library analysis reveals an unusual composition and strong habitat partitioning of pelagic bacterial communities in Lake Tanganyika

Aaike De Wever1,*, Katleen Van der Gucht1, Koenraad Muylaert2, Sylvie Cousin1, Wim Vyverman1

1Ghent University, Department of Biology, Section of Protistology and Aquatic Ecology, Krijgslaan 281 S8, 9000 Ghent, Belgium
2K. U. Leuven–Campus Kortrijk, E. Sabbelaan 53, 8500 Kortrijk, Belgium

ABSTRACT: The phylogenetic composition of bacterioplankton communities in Lake Tanganyika was studied by sequencing 16S rRNA gene clones. Four clone libraries were constructed from oxic epilimnion and anoxic hypolimnion samples collected during the dry season of 2002 in the northern and southern basins. Clone library analysis revealed a bacterial community composition (BCC) differing from previously studied freshwater systems and clear differences between both epi- and hypolimnion and the northern and southern basins. We detected few representatives of the Actinobacteria, Bacteroidetes, Cyanobacteria, and Alpha- and Betaproteobacteria commonly found in freshwater environments in temperate and cold regions, but observed a remarkably high number of clones belonging to Chloroflexi and Gammaproteobacteria. This was especially the case in the hypolimnion, but also in the epilimnion in the south of the lake, which suggests that the BCC may be influenced by seasonal upwelling. In total, nearly half of the detected operational taxonomical units were not closely related to bacteria previously observed in freshwater environments. Even in the epilimnetic clone libraries, genotypes commonly reported from oxic freshwater environments (e.g. ACK4, LD12, Sta2-30) were rare or absent.


KEY WORDS: Bacterial community composition · Clone library · Lake Tanganyika · Freshwater · Tropical lake


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Cite this article as: De Wever A, Van der Gucht K, Muylaert K, Cousin S, Vyverman W (2008) Clone library analysis reveals an unusual composition and strong habitat partitioning of pelagic bacterial communities in Lake Tanganyika. Aquat Microb Ecol 50:113-122. https://doi.org/10.3354/ame01157

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