AME 51:129-140 (2008)  -  DOI: https://doi.org/10.3354/ame01186

Habitat partitioning of denitrifying bacterial communities carrying nirS or nirK genes in the stratified water column of Lake Kinneret, Israel

Pilar Junier1,2,*, Ok-Sun Kim2, Karl-Paul Witzel2, Johannes F. Imhoff3, Ora Hadas4

1École Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland
2Max Planck Institute for Evolutionary Biology, 24306 Plön, Germany
3Leibniz Institute of Marine Sciences, University of Kiel, 24105 Kiel, Germany
4Israel Oceanographic and Limnological Research, Kinneret Limnological Laboratory, 14950 Migdal, Israel

ABSTRACT: The community composition of denitrifying bacteria was studied in the stratified water column of Lake Kinneret. The nitrite reductase genes nirS and nirK were amplified by PCR from water samples taken at 1, 14, 19 and 22 m depth, which represent the epi-, meta- and hypolimnion of the lake. The PCR products were analyzed with terminal restriction fragment length polymorphism (T-RFLP) and clone libraries. The highest diversity of nirS denitrifying communities was observed at 1 m depth. According to the T-RFLP profiles and clone libraries of nirS products, 2 groups of denitrifiers were common to and dominant in all depths. Deduced protein sequences from one of these groups displayed low identity (77%) with other nirS sequences reported in GenBank. Denitrifying bacterial communities with nirK were most diverse at 22 m and showed highest similarity to those at 19 m depth. Sequences unrelated to nirK dominated the clone libraries from 1 m depth, suggesting that denitrifying bacteria with copper-containing nitrite reductase were less frequent at this depth. The results suggest that microorganisms with nirK and those with nirS respond differently to the environmental conditions in the stratified water column of Lake Kinneret.


KEY WORDS: nirS · nirK · T-RFLP · Lake Kinneret


Full text in pdf format 
Cite this article as: Junier P, Kim OS, Witzel KP, Imhoff JF, Hadas O (2008) Habitat partitioning of denitrifying bacterial communities carrying nirS or nirK genes in the stratified water column of Lake Kinneret, Israel. Aquat Microb Ecol 51:129-140. https://doi.org/10.3354/ame01186

Export citation
Mail this link - Contents Mailing Lists - RSS
- -