AME 54:163-170 (2009)  -  DOI: https://doi.org/10.3354/ame01266

Isolation of a novel cyanophage infectious to the filamentous cyanobacterium Planktothrix agardhii (Cyanophyceae) from Lake Donghu, China

E-Bin Gao, Xiu-Ping Yuan, Ren-hui Li, Qi-Ya Zhang*

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Graduate University of the Chinese Academy of Sciences, Wuhan 430072, PR China
*Corresponding author. Email:

ABSTRACT: A previously unknown cyanophage, PaV-LD (Planktothrix agardhii Virus isolated from Lake Donghu), which causes lysis of the bloom-forming filamentous cyanobacterium P. agardhii, was isolated from Lake Donghu, Wuhan, China. PaV-LD only lysed P. agardhii strains isolated from Lake Donghu and not those isolated from other lakes. The PaV-LD particle has an icosahedral, non-tailed structure, ca. 70 to 85 nm (mean ± SD = 76 ± 6 nm) in diameter. PaV-LD was stable at freezing temperature, but lost its infectivity at temperatures >50°C. Lysis of host cells was delayed about 3 d after the PaV-LD treatment with chloroform, and the virus was inactivated by exposure to low pH (≤4). The latent period and burst size of the PaV-LD were estimated to be 48 to 72 h and about 340 infectious units per cell, respectively. The regrowth cultures of surviving host filaments were not lysed by the PaV-LD suspension. To our knowledge, this is the first isolation and cultivation of a virus infectious to the filamentous bloom-forming cyanobacterium Planktothrix from a freshwater lake.


KEY WORDS: Cyanophage · Planktothrix agardhii · Viral infection · Blooms · Cyanobacteria · Freshwater lake


Full text in pdf format 
Cite this article as: Gao EB, Yuan XP, Li RH, Zhang QY (2009) Isolation of a novel cyanophage infectious to the filamentous cyanobacterium Planktothrix agardhii (Cyanophyceae) from Lake Donghu, China. Aquat Microb Ecol 54:163-170. https://doi.org/10.3354/ame01266

Export citation
Mail this link - Contents Mailing Lists - RSS
- -