AME 56:263-273 (2009)  -  DOI: https://doi.org/10.3354/ame01326

Oxygen evolution in a hypersaline crust: in situ photosynthesis quantification by microelectrode profiling and use of planar optode spots in incubation chambers

Jana Woelfel1, Ketil Sørensen2, Mareike Warkentin1, Stefan Forster1, Aharon Oren3, Rhena Schumann1,*

1University of Rostock, Institute for Biological Sciences, Albert- Einstein-Straße 3, 18059 Rostock, Germany
2Danish Technological Institute, Kongsrang Allé 29, 8000 Aarhus, Denmark
3The Institute of Life Sciences, and the Moshe Shilo Minerva Center for Marine Biogeochemistry, The Hebrew University of Jerusalem, Jerusalem, Israel
*Corresponding author. Email:

ABSTRACT: Net primary production and respiration were estimated in a hypersaline cyanobacterial mat colonizing a gypsum crust in the Eilat salterns, Israel. Two different approaches were used: in situ microprofiling with Clark-type O2 sensors and application of optode sensor spots in incubation chambers. The net O2 release rates of the mat phototrophs was high, with a maximum of 3.4 nmol O2 cm–2 min–1 measured by microprofiling and 4.4 nmol O2 cm–2 min–1 determined in the incubation chambers. The upper 2 layers of the mat as well as the overlying water quickly became O2 saturated during the day. The respiration of the whole gypsum crust was also very intensive and corresponded to the O2 produced by photosynthesis on a diurnal basis, which prevented most of the evolved O2 from reaching the water. The results presented show that optode sensor spots are useful tools providing additional information about export and photosynthetic production rates of O2 in hypersaline microbial mats.


KEY WORDS: Hypersaline · Gypsum crust · Microbial mat · Net production and respiration · Microelectrode · Optode


Full text in pdf format  
Cite this article as: Woelfel J, Sørensen K, Warkentin M, Forster S, Oren A, Schumann R (2009) Oxygen evolution in a hypersaline crust: in situ photosynthesis quantification by microelectrode profiling and use of planar optode spots in incubation chambers. Aquat Microb Ecol 56:263-273. https://doi.org/10.3354/ame01326

Export citation
Mail this link - Contents Mailing Lists - RSS
- -