AME 62:13-24 (2011)  -  DOI: https://doi.org/10.3354/ame01432

Changes in eukaryotic microbial communities of vernal pools along an urban–rural land use gradient

Sarah R. Carrino-Kyker1,*, Andrew K. Swanson2, David J. Burke1,3 

1Department of Biology, Case Western Reserve University, 2080 Adelbert Road, Cleveland, Ohio 44106-7080, USA
2Phycal LLC, 51 Alpha Park, Highland Heights, Ohio 44143, USA
3The Holden Arboretum, 9500 Sperry Road, Kirtland, Ohio 44094, USA

ABSTRACT:  Urban–rural gradients are important ecological settings to investigate how human land use impacts habitats and, indirectly, inherent organisms. Seasonally flooded vernal pools are common throughout temperate forest landscapes and may represent significant sites of forest nutrient cycling. The effects of urbanization on vernal pool microbial communities, important drivers of nutrient cycling, are largely unknown; thus studies to improve our understanding of microbes and their functional roles in these habitats are needed. Eukaryotic microbial communities sampled from 30 vernal pools of the Cuyahoga River watershed (USA), located along a gradient of urban land use, were profiled with denaturing gradient gel electrophoresis, and were compared between pools using nonmetric multidimensional scaling (NMS). Microbial diversity, and, specifically, the richness and diversity of the fungal operational taxonomic units (OTUs), increased with urbanization. Vernal pool eukaryotic microorganisms formed 2 NMS clusters that differed significantly in sub-watershed urban area. However, the significance of urbanization disappeared when fungal and algal communities were analyzed separately. Water conductivity was consistently correlated with different microbial communities (e.g. eukaryotic, fungal, and algal). Fungal communities also appeared related to the carbon content of the substrate, indicating that vegetation at a local scale may be important for community structure. Almost half of the OTUs matched fungal species, which provides taxonomic evidence that the eukaryotic microbial communities of vernal pools are dominated by fungal species. Overall, our data suggest that the eukaryotic microbes of vernal pools are influenced by a variety of factors of the surrounding landscape, including urbanization, water chemistry, and vegetation type.


KEY WORDS: 18S rDNA . Denaturing gradient gel electrophoresis . Diversity . Microbial communities . Vernal pools . Urbanization . Litter quality . Richness


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Cite this article as: Carrino-Kyker SR, Swanson AK, Burke DJ (2011) Changes in eukaryotic microbial communities of vernal pools along an urban–rural land use gradient. Aquat Microb Ecol 62:13-24. https://doi.org/10.3354/ame01432

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