AME 71:91-97 (2013)  -  DOI: https://doi.org/10.3354/ame01665

Composition of carbon, nitrogen and phosphorus in single cells of three filamentous cyanobacteria using nuclear microprobe and traditional techniques

Eva Pérez Blanco1,*, Chatarina Karlsson1, Jan Pallon2, Edna Granéli1

1LnUCEEMiS - School of Natural Sciences, Linnaeus University, 391 82 Kalmar, Sweden
2Division of Nuclear Physics, Department of Physics, Lund University, Box 118, 221 00 Lund, Sweden

ABSTRACT: Intracellular of carbon, nitrogen and phosphorus content in phytoplankton cells is traditionally measured using concentrates containing thousands to millions of cells. In the present study, we used a nuclear microprobe (NMP) to determine C, N and P concentrations in single filaments of 3 cyanobacteria species: Anabaena sp., Nodularia spumigena and Aphanizomenon flos-aquae var. klebahnii isolated from Baltic Sea water. Estimations of C, N and P content per cell were calculated and compared to the concentrations found using traditional bulk methods. No significant differences regarding the C, N and P cellular content were found between the 2 methods for each of the species tested (p < 0.05). From our results, we conclude that the NMP can be a useful tool for studying the elemental content of single phytoplankton cells.


KEY WORDS: Baltic Sea · Elemental cellular content · Nitrogen-fixing cyanobacteria · Nuclear microprobe · Nutrients


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Cite this article as: Pérez Blanco E, Karlsson C, Pallon J, Granéli E (2013) Composition of carbon, nitrogen and phosphorus in single cells of three filamentous cyanobacteria using nuclear microprobe and traditional techniques. Aquat Microb Ecol 71:91-97. https://doi.org/10.3354/ame01665

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