AME 75:117-128 (2015)  -  DOI: https://doi.org/10.3354/ame01752

Metagenomic assessment of viral diversity in Lake Matoaka, a temperate, eutrophic freshwater lake in southeastern Virginia, USA

Jasmin C. Green1, Faraz Rahman1, Matthew A. Saxton2,3, Kurt E. Williamson1,2,*

1Department of Biology, College of William & Mary, Williamsburg, Virginia 23185, USA
2Environmental Science and Policy Program, College of William & Mary, Williamsburg, Virginia 23185, USA
3Present address: Department of Marine Sciences, University of Georgia, Athens, Georgia 30602, USA
*Corresponding author:

ABSTRACT: Little is known about the composition and diversity of temperate freshwater viral communities. This study presents a metagenomic analysis of viral community composition, taxonomic and functional diversity of temperate, eutrophic Lake Matoaka in southeastern Virginia (USA). Three sampling sites were chosen to represent differences in anthropogenic impacts: the Crim Dell Creek mouth (impacted), the Pogonia Creek mouth (less impacted) and the main body of the lake (mixed). Sequences belonging to tailed bacteriophages were the most abundant at all 3 sites, with Podoviridae predominating. The main lake body harbored the highest virus genotype richness and included cyanophage and eukaryotic algal virus sequences not found at the other 2 sites, while the impacted Crim Dell Creek mouth showed the lowest richness. Cross-contig comparisons indicated that similar virus genotypes were found at all 3 sites, but at different rank-abundances. Hierarchical cluster analysis of multiple viral metagenomes indicated high genetic similarity between viral communities of related environments, with freshwater, marine, hypersaline, and eukaryote-associated environments forming into clear groups despite large geographic distances between sampling locations within each environment type. These results support the conclusion that freshwater viral communities are genetically distinct from virus assemblages in other environments.


KEY WORDS: Virus · Freshwater · Lake · Temperate · Metagenome · Sequencing · Virome


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Cite this article as: Green JC, Rahman F, Saxton MA, Williamson KE (2015) Metagenomic assessment of viral diversity in Lake Matoaka, a temperate, eutrophic freshwater lake in southeastern Virginia, USA. Aquat Microb Ecol 75:117-128. https://doi.org/10.3354/ame01752

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