AME - Vol. 78 No. 2 - Feature article

Diatom cells (large) releasing Alcian blue-stained transparent exopolymeric particles (blue) and surrounded by Marinobacter adhaerens cells (rod-shaped) (Photo: Veronika Will).

Stahl A, Ullrich MS

 

Proteomics analysis of the response of the marine bacterium Marinobacter adhaerens HP15 to the diatom Thalassiosira weissflogii

 

In-depth cell-to-cell studies on marine bacteria–diatom interactions remain scarce. Proteomics was applied to a bilateral model system consisting of Marinobacter adhaerens and Thalassiosira weissflogii. Observed protein alterations gave information on substrates that determine this potential mutualistic interaction. Results suggested a differentiated nutrient supply for M. adhaerens during co-cultivation. The bacterium seemed to benefit from the release of certain amino acids by the diatom as indicated by an up-regulation of several transporter elements responsible for amino acid uptake. To substantiate this, nutrient substrates of M. adhaerens were screened revealing amino acids but not sugars as preferred carbon sources of the bacterium. In future, an in-depth proteome analysis of the diatom will be conducted to gain more insight into this fascinating interaction.

 

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