CR 43:41-47 (2010)  -  doi:10.3354/cr00896

Genetic variation in heat resistance and HSP70 expression in inbred isofemale lines of the springtail Orchesella cincta

S. Bahrndorff1,3,4,*, J. Mariën2, V. Loeschcke1, J. Ellers2

1Ecology and Genetics, Department of Biological Sciences, Aarhus University, Ny Munkegade, Building 1540,
8000 Aarhus C, Denmark
2Department of Animal Ecology, Institute of Ecological Science, Faculty of Earth and Life Sciences, VU University Amsterdam, De Boelelaan 1085, 1081 HV, Amsterdam, The Netherlands
3Natural History Museum, Wilhelm Meyers Allé 210, 8000 Aarhus C, Denmark
4Knowledge Centre for Agriculture, Agro Food Park 15, 8200 Århus N, Denmark

ABSTRACT: The functional relationship between thermotolerance and heat shock protein (HSP) expression is well described. However, the intraspecific variation in HSP70 expression and heat resistance is not fully understood. Some studies have shown a correlation between thermotolerance and HSP70 expression levels at the intraspecific level, whereas others have not, and results depend on species, developmental stage and environment. The present study extends such correlative studies to species from soil ecosystems, where we have characterized the variation in thermotolerance and HSP70 expression in 18 inbred isofemale lines of the springtail Orchesella cincta. The results show ample genetic variation among lines in heat shock resistance as well as in HSP70 protein levels and hsp70 mRNA expression. However, we did not detect any significant positive correlations between HSP70 expression and thermotolerance. These results indicate that the variation in HSP70 expression and heat resistance at the intraspecific level is dependent on species and/or ecosystem and further studies are needed to clarify this relationship.


KEY WORDS: Heat shock resistance · Collembola · Thermotolerance · Thermal adaptation · Climate change · Evolution


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Cite this article as: Bahrndorff S, Mariën J, Loeschcke V, Ellers J (2010) Genetic variation in heat resistance and HSP70 expression in inbred isofemale lines of the springtail Orchesella cincta. Clim Res 43:41-47

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