DAO 100:83-88 (2012)  -  DOI: https://doi.org/10.3354/dao02478

NOTE
First identification of Flavobacterium columnare infection in farmed freshwater striped catfish Pangasianodon hypophthalmus

Nguyen Thi Tien1, Tu Thanh Dung1, Nguyen Anh Tuan1, Mags Crumlish2,*

1College of Aquaculture Fisheries, Cantho University, Cantho City, Vietnam
2Institute of Aquaculture, Stirling University, Stirling FK9 4LA, UK
*Corresponding author. Email:

ABSTRACT: The bacterium Flavobacterium columnare was recovered and identified as the aetiological agent causing freshwater columnaris infection in farmed striped catfish Pangasianodon hypophthalmus (Sauvage) fingerlings that had suffered high mortality rates within commercial hatchery ponds in Vietnam. The gross clinical signs were typical of columnaris-infected fish. Histological examination found numerous Gram-negative, filamentous bacteria present on the skin, muscle and gill tissues of affected fish. The yellow-pigmented bacteria were isolated and identified as F. columnare using primary, biochemical and PCR methods. An experimental immersion-challenge study with 2 strains was also performed. It fulfilled Koch’s postulates and showed a median lethal concentration (LC50) of 4.27 × 105 and 1.66 × 106 cfu ml−1 for the F. columnare strains FC-HN and FC-CT, respectively. To the best of our knowledge this is the first report of freshwater columnaris infection in P. hypophthalmus.


KEY WORDS: Flavobacterium columnare · White patch disease · Pangasianodon hypophthalmus


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Cite this article as: Tien NT, Dung TT, Tuan NT, Crumlish M (2012) First identification of Flavobacterium columnare infection in farmed freshwater striped catfish Pangasianodon hypophthalmus. Dis Aquat Org 100:83-88. https://doi.org/10.3354/dao02478

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