DAO 101:167-171 (2012)  -  DOI: https://doi.org/10.3354/dao02511

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Effects of high salinity, high temperature and pH on capsid structure of white spot syndrome virus

Weiyu Chen1,2,*, Heng Zhang2,*, Li Gu2, Fang Li2, Feng Yang2,**

1College of Oceanography and Environmental Science of Xiamen University, Xiamen 361005, PR China
2Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Xiamen 361005, PR China
*These authors contributed equally to this work
**Corresponding author. Email:

ABSTRACT: The structural stability of white spot syndrome virus (WSSV) capsids at high salinity, high temperature and various pH values was studied. To obtain the viral capsids, the nucleocapsids were treated with high salinity. The results showed that high salinity treatment can cause the dissociation of VP15 and most of VP95 from the nucleocapsid, but there were no noticeable alterations in morphology and ultrastructure of the nucleocapsid and capsid. The capsids retained morphological integrity at temperatures <45°C but became aberrant at >60°C. In addition, the capsids were relatively resistant to strong acid conditions and were tolerant to a broad pH range of 1 to 10. However, morphological change occurred at pH 10.5. The capsids broke up into small pieces at pH 11 and completely degraded in 0.1 and 1.0 M NaOH. These results indicated that the WSSV capsid is acid-stable and alkali-labile.


KEY WORDS: WSSV · Capsid · Transmission electron microscopy · Structural stability


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Cite this article as: Chen W, Zhang H, Gu L, Li F, Yang F (2012) Effects of high salinity, high temperature and pH on capsid structure of white spot syndrome virus. Dis Aquat Org 101:167-171. https://doi.org/10.3354/dao02511

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