DAO 104:197-202 (2013)  -  DOI: https://doi.org/10.3354/dao02590

Evidence for two koi herpesvirus (KHV) genotypes in South Korea

Hyoung Jun Kim1, Se Ryun Kwon2,*

1Animal, Plant and Fisheries Quarantine and Inspection Agency, Jungbu Regional Office, Incheon 400-800, South Korea
2Department of Aquatic Life Medical Sciences, Sunmoon University, Asan-si, Chungnam 336-708, South Korea
*Corresponding author. Email:

ABSTRACT: The geographic distribution of koi herpesvirus (KHV) has recently been analyzed by polymerase chain reaction (PCR, based on the alleles of 3 domains) and sequence analysis using 3 regions of KHV genomic DNA (SphI-5, 9/5, and the thymidine kinase gene). In this study, samples from 6 carp showing symptoms of KHV infection in 2008 were examined for the presence of KHV by using PCR and cell culture isolation methods. KHV was detected in 2 (Pyeongtaek and Buan) of the samples. Sequence analysis revealed that the genotype of the KHV PT-08 isolate was Asia genotype variant 1 (A1), and the genotype of the KHV BA-08 isolate was European genotype variant 4 (E4). In addition, PCR patterns and sequence analysis based on the alleles of 3 domains of an alternate KHV classification system confirmed that the genotype of the KHV PT-08 isolate was CyHV3-J, and the genotype of the KHV BA-08 isolate was CyHV3-third genotype. To our knowledge, this is the first study to demonstrate the presence of 2 genotypes of KHV (genotype A1/CyHV3-J; genotype E4/CyHV3-third genotype) in South Korea.


KEY WORDS: Koi herpesvirus · Genotype · Sequencing analysis · Korea


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Cite this article as: Kim HJ, Kwon SR (2013) Evidence for two koi herpesvirus (KHV) genotypes in South Korea. Dis Aquat Org 104:197-202. https://doi.org/10.3354/dao02590

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