DAO 104:203-214 (2013)  -  DOI: https://doi.org/10.3354/dao02587

Complete genome sequence and transcription profiles of the rock bream iridovirus RBIV-C1

Bao-Cun Zhang1,2, Min Zhang1, Bo-Guang Sun1, Yong Fang1,2, Zhi-Zhong Xiao1, Li Sun1,*

1Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China
2Graduate University of the Chinese Academy of Sciences, Beijing 100049, PR China
*Corresponding author. Email:

ABSTRACT: The family Iridoviridae consists of 5 genera of double-stranded DNA viruses, including the genus Megalocytivirus, which contains species that are important fish pathogens. In a previous study, we isolated the first rock bream iridovirus from China (RBIV-C1) and identified it as a member of the genus Megalocytivirus. In this report, we determined the complete genomic sequence of RBIV-C1 and examined its in vivo expression profiles. The genome of RBIV-C1 is 112333 bp in length, with a GC content of 55% and a coding density of 92%. RBIV-C1 contains 4584 simple sequence repeats, 89.8% of which are distributed among coding regions. A total of 119 potential open reading frames (ORFs) were identified in RBIV-C1, including the 26 core iridovirus genes; 41 ORFs encode proteins that are predicted to be associated with essential biological functions. RBIV-C1 exhibits the highest degree of sequence conservation and colinear arrangement of genes with orange-spotted grouper iridovirus (OSGIV) and rock bream iridovirus (RBIV). The pairwise nucleotide identities are 99.49% between RBIV-C1 and OSGIV and 98.69% between RBIV-C1 and RBIV. Compared to OSGIV, RBIV-C1 contains 11 insertions, 13 deletions, and 103 single nucleotide mutations. Whole-genome transcription analysis showed that following experimental infection of rock bream with RBIV-C1, all but 1 of the 119 ORFs were expressed at different time points and clustered into 3 hierarchical groups based on their expression patterns. These results provide new insights into the genetic nature and gene expression features of megalocytiviruses.


KEY WORDS: Iridovirus · Megalocytivirus · Comparative genome · Oplegnathus fasciatus


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Cite this article as: Zhang BC, Zhang M, Sun BG, Fang Y, Xiao ZZ, Sun L (2013) Complete genome sequence and transcription profiles of the rock bream iridovirus RBIV-C1. Dis Aquat Org 104:203-214. https://doi.org/10.3354/dao02587

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