DAO 105:81-87 (2013)  -  DOI: https://doi.org/10.3354/dao02612

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Cytomorphology and PCNA expression pattern in bivalves Mytilus galloprovincialis and Cerastoderma edule with haemic neoplasia

Francesca Carella1,*, Antonio Figueras2, Beatriz Novoa2, Gionata De Vico

1University of Naples Federico II, Department of Biology, Via Mezzocannone 8, 80134 Naples, Italy
2Instituto de Investigaciones Marinas (IIM), CSIC, Eduardo Cabello 6, 36208 Vigo, Spain

ABSTRACT: Haemic neoplasia (HN) is a pathologic condition reported in several bivalve species in different geographic areas. In this study we describe the cytomorphological features and the proliferative behaviour, assessed by the proliferating cell nuclear antigen (PCNA), of HN in common cockle Cerastoderma edule and Mediterranean mussel Mytilus galloprovicialis. In mussels the presence of at least 5 types of atypical haemocytes was detected, including A- and B-type cells, previously described in M. edulis and Mytilus sp., with predominance of A-type cells in early phases of the disease and B-type cells in more advanced stages. PCNA immunostaining was positive for 97 to 100% of the neoplastic cells, with both cytoplasmic (A cells) and nuclear patterns (B cells). Conversely, in C. edule there was no distinctive morphological cell sub-population, and staining atypical haemocytes with PCNA (range 93 to 100%) showed nuclear expression in early phases of disease and cytoplasmic expression in more advanced stages. The above findings suggest distinct histo-pathogenetic pathways for HN in mussels and common cockles.


KEY WORDS: Mediterranean mussel · Common cockle · Haemic neoplasia · Proliferating nuclear antigen · Proliferation indices


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Cite this article as: Carella F, Figueras A, Novoa B, De Vico G (2013) Cytomorphology and PCNA expression pattern in bivalves Mytilus galloprovincialis and Cerastoderma edule with haemic neoplasia. Dis Aquat Org 105:81-87. https://doi.org/10.3354/dao02612

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