DAO 106:39-47 (2013)  -  DOI: https://doi.org/10.3354/dao02617

Protection against Vibrio alginolyticus in crimson snapper Lutjanus erythropterus immunized with a DNA vaccine containing the ompW gene

Shuang-Hu Cai1,2,3, Yi-Shan Lu1,2,3, Ji-Chang Jian1,2,3, Bei Wang1,2,3, Yu-Cong Huang1,2,3, Ju-Fen Tang1,2,3, Yu Ding1,2,3, Zao-He Wu2,3,4,*

1Fisheries College, Guangdong Ocean University, Zhanjiang 524088, PR China
2Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524088, PR China
3Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang 524088, PR China
4Zhongkai University of Agriculture and Engineering, Guangzhou 510225, PR China
*Corresponding author. Email:

ABSTRACT: The outer membrane proteins of Vibrio alginolyticus play an important role in the virulence of the bacterium and are potential candidates for vaccine development. In the present study, the ompW gene was cloned, expressed and purified. A DNA vaccine was constructed by inserting the ompW gene into a pcDNA plasmid. Crimson snapper Lutjanus erythropterus (Bloch) were injected intramuscularly with the recombinant plasmid pcDNA-ompW. The expression of the DNA vaccine was detected in gill, head kidney, heart, liver, spleen and injection site muscle of crimson snapper by RT-PCR 7 and 28 d post-vaccination. The ELISA results demonstrated that the DNA vaccine produced an observable antibody response in all sera of the vaccinated fish. In addition, crimson snapper immunized with the DNA vaccine showed a relative percentage survival (RPS) of 92.53%, indicating effective protection against V. alginolyticus infection.


KEY WORDS: Vibriosis · Vaccination · Recombinant plasmid · Outer membrane protein · pcDNA · Aquaculture


Full text in pdf format 
Cite this article as: Cai SH, Lu YS, Jian JC, Wang B and others (2013) Protection against Vibrio alginolyticus in crimson snapper Lutjanus erythropterus immunized with a DNA vaccine containing the ompW gene. Dis Aquat Org 106:39-47. https://doi.org/10.3354/dao02617

Export citation
Mail this link - Contents Mailing Lists - RSS
- -