DAO 107:211-221 (2014)  -  DOI: https://doi.org/10.3354/dao02685

Henneguya cuniculator sp. nov., a parasite of spotted sorubim Pseudoplatystoma corruscans in the São Francisco Basin, Brazil

Juliana Naldoni1, Antônio A. M. Maia2,*, Marcia R. M. da Silva2, Edson A. Adriano1,3 

1Departamento de Biologia Animal da Universidade Estadual de Campinas (UNICAMP), Rua Monteiro Lobato, 255, CEP 13083-970, Campinas, Sao Paulo, Brazil
2Departamento de Ciências Básicas, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo (USP), Rua Duque de Caxias Norte, 225, CEP 13635-900, Pirassununga, Sao Paulo, Brazil
3Departamento de Ciências Biológicas, Universidade Federal de São Paulo (UNIFESP), Rua Professor Artur Riedel, 275, Jardim Eldorado, CEP 09972-270, Diadema, Sao Paulo, Brazil
*Corresponding author:

ABSTRACT: Henneguya cuniculator sp. nov. was found infecting spotted sorubim catfish Pseudoplatystoma corruscans from the São Francisco River, Minas Gerais, Brazil. The parasites form elongated plasmodia of up to 1 cm in length in the gill filaments. Mature spores were ellipsoidal from the frontal view, with total length of 29.4 ± 2.4 (mean ± SD, range 23.3-32.4) µm, body length of 12.1 ± 1.0 (10.0-14.7) µm, width of 4.8 ± 0.4 (4.0-5.9) µm, and tail length of 16.7 ± 2.0 (12.3-19.4) µm. From the lateral view, spores were biconvex, with thickness of 4.2 ± 0.7 (3.9-4.9) µm. The polar capsules were elongated and equal in size, 6.2 ± 0.3 (5.2-6.2) µm in length, and 1.8 ± 0.1 (1.4-1.9) µm in width. Ultrastructural analysis showed that the plasmodial wall had delicate projections towards the host tissue and a thin layer that prevented contact between the host cells and the parasite. In the ectoplasm, few mitochondria were observed, while generative cells, early stages of sporogenesis, and advanced spore development occurred in the plasmodial periphery, and more mature spores in internal regions. Histopathological analysis showed that plasmodia developed in the sub-epithelial connective tissue of gill filaments, causing compression of the adjacent tissues, deformation of gill filaments, and lamellar fusion. Phylogenetic analysis, based on 18S rDNA genes and using only Henneguya/Myxobolus species parasites of siluriform fish, showed grouping according to the fish family.


KEY WORDS: Myxozoa · Pseudoplatystoma · São Francisco River · 18S rDNA · Ultrastructure


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Cite this article as: Naldoni J, Maia AAM, Silva MRM, Adriano EA (2014) Henneguya cuniculator sp. nov., a parasite of spotted sorubim Pseudoplatystoma corruscans in the São Francisco Basin, Brazil. Dis Aquat Org 107:211-221. https://doi.org/10.3354/dao02685

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