DAO 112:139-148 (2014)  -  DOI: https://doi.org/10.3354/dao02794

Ultrastructure and phylogeny of the parasite Henneguya carolina sp. nov. (Myxozoa), from the marine fish Trachinotus carolinus in Brazil

S. Rocha1,2, G. Casal1,3, P. Garcia4, E. Matos5, S. Al-Quraishy6, C. Azevedo1,2,6,*

1Laboratory of Animal Pathology, Interdisciplinary Centre of Marine and Environmental Research (CIIMAR), University of Porto, 4050-123 Porto, Portugal
2Laboratory of Cell Biology, Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, 4050-313 Porto, Portugal
3Department of Sciences, High Institute of Health Sciences-North (CESPU), 4585-116 Gandra, Portugal
4AQUOS-Aquatic Organism Health Laboratory, Aquaculture Department, Federal University of Santa Catarina (UFSC), 88040-900 Florianópolis, SC, Brazil
5Carlos Azevedo Research Laboratory, Federal Rural University of Amazonia (UFRA), 66077-901 Belém, PA, Brazil
6Zoology Department, College of Sciences, King Saud University, 11451 Riyadh, Saudi Arabia
*Corresponding author:

ABSTRACT: Microscopic and molecular procedures are used to describe a new myxosporean species, Henneguya carolina sp. nov., found infecting the intestine of the marine teleost fish Trachinotus carolinus on the southern Atlantic coast of Brazil. Spherical to ellipsoid cysts, measuring up to ~750 µm, display synchronous development. Mature myxospores are ellipsoidal with a bifurcated caudal process. Myxospore body length, width, and thickness are 12.7 ± 0.8 (12.0-13.4) µm, 8.8 ± 0.6 (7.5-9.6) µm, and 5.8 ± 0.4 (5.0-6.4) µm, respectively; 2 equal caudal processes are 16.8 ± 1.1 (15.9-18.0) µm long, and the total myxospore length is 29.4 ± 0.8 (28.4-30.4) µm. Two pyriform polar capsules measure 5.0 ± 0.5 (4.6-5.6) × 2.4 ± 0.4 (1.9-2.9) µm, and each contains a polar filament forming 3 to 4 coils. Sporoplasm is binucleated and presents a spherical vacuole surrounded by numerous globular sporoplasmosomes. Molecular analysis of the small subunit rRNA gene by maximum parsimony, neighbor joining, and maximum likelihood reveals the parasite clustering together with other myxobolids that are histozoic in marine fish of the order Perciformes, thereby strengthening the contention that the host phylogenetic relationships and aquatic environment are the strongest evolutionary signal for myxosporeans of the family Myxobolidae.


KEY WORDS: Myxozoa · Florida pompano · Fish-infecting · Marine · Perciformes · Intestine · Ultrastructure · SSU rRNA · Host affinity


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Cite this article as: Rocha S, Casal G, Garcia P, Matos E, Al-Quraishy S, Azevedo C (2014) Ultrastructure and phylogeny of the parasite Henneguya carolina sp. nov. (Myxozoa), from the marine fish Trachinotus carolinus in Brazil. Dis Aquat Org 112:139-148. https://doi.org/10.3354/dao02794

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