DAO 114:127-137 (2015)  -  DOI: https://doi.org/10.3354/dao02852

Occurrence of antibiotic resistance genes in culturable bacteria isolated from Turkish trout farms and their local aquatic environment

Erol Capkin1, Ertugrul Terzi2, Ilhan Altinok1,*

1Karadeniz Technical University, Faculty of Marine Science, Department of Fisheries Technology Engineering, 61530 Surmene, Trabzon, Turkey
2RTE University, Faculty of Fisheries, 53100 Rize, Turkey
*Corresponding author:

ABSTRACT: Antibiotic resistance and presence of the resistance genes were investigated in the bacteria isolated from water, sediment, and fish in trout farms. A total of 9 bacterial species, particularly Escherichia coli, were isolated from the water and sediment samples, and 12 species were isolated from fish. The antimicrobial test indicated the highest resistance against sulfamethoxazole and ampicillin in coliform bacteria, and against sulfamethoxazole, imipenem, and aztreonam in known pathogenic bacteria isolated from fish. The most effective antibiotics were rifampicin, chloramphenicol, and tetracycline. The multiple antibiotic resistance index was above the critical limit for almost all of the bacteria isolated. The most common antibiotic resistance gene was ampC, followed by tetA, sul2, blaCTX-M1, and blaTEM in the coliform bacteria. At least one resistance gene was found in 70.8% of the bacteria, and 66.6% of the bacteria had 2 or more resistance genes. Approximately 36.54% of the bacteria that contain plasmids were able to transfer them to other bacteria. The plasmid-mediated transferable resistance genes were ampC, blaCTX-M1, tetA, sul2, and blaTEM. These results indicate that the aquatic environment could play an important role in the development of antibiotic resistance and the dissemination of resistance genes among bacteria.


KEY WORDS: Antibiotic resistance gene · Coliform bacteria · Fish pathogen · Antimicrobials · Multiple antibiotic resistance index


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Cite this article as: Capkin E, Terzi E, Altinok I (2015) Occurrence of antibiotic resistance genes in culturable bacteria isolated from Turkish trout farms and their local aquatic environment. Dis Aquat Org 114:127-137. https://doi.org/10.3354/dao02852

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