DAO 115:25-36 (2015)  -  DOI: https://doi.org/10.3354/dao02872

Goldfish Carassius auratus susceptibility to viral hemorrhagic septicemia virus genotype IVb depends on exposure route

Rodman G. Getchell1,*, Toni Erkinharju1,2, Anna O. Johnson1,3, Benjamin W. Davis1, Emily E. Hatch1,4, Emily R. Cornwell1, Paul R. Bowser1

1Aquatic Animal Health Program, Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, 930 Campus Road, Ithaca, NY 14853, USA
2Present address: Faculty of Biosciences, Fisheries and Economics, Norwegian College of Fishery Science, University of Tromsø, Pb 6050 Langnes, 9037 Tromsø, Norway
3Present address: Department of Biology, College of Charleston, 66 George Street, Charleston, SC 29424, USA
4Present address: Department of Education/Childhood Education, Corning Community College, 1 Academic Drive, Corning, NY 14830, USA
*Corresponding author:

ABSTRACT: We assessed the susceptibility of goldfish Carassius auratus to infection by genotype IVb of the viral hemorrhagic septicemia virus. Goldfish were infected by intraperitoneal injections of 106 plaque-forming units (pfu) fish-1, single bath exposure of 105 pfu ml-1 for 24 h, or consumption of 0.4 g of commercial fish feed soaked in 107 pfu per 8 fish. The mortality rate of intraperitoneal-infected goldfish was 10 to 32%, although the virus was detected by quantitative RT-PCR in 77% (65/84) of the survivors at the end of the 42 d trial, suggesting a carrier state. Severe gross lesions were observed in many of the moribund and dead goldfish such as hemorrhaging in the skin, fin, liver, kidney, brain, intestine, and eye as well as abdominal distension, bilateral exophthalmia, and splenomegaly. There was minimal morbidity or mortality in the immersion, feeding, or control groups.


KEY WORDS: Carassius auratus · Infection route · Cyprinid · Viral haemorrhagic septicemia virus · VHSV IVb


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Cite this article as: Getchell RG, Erkinharju T, Johnson AO, Davis BW, Hatch EE, Cornwell ER, Bowser PR (2015) Goldfish Carassius auratus susceptibility to viral hemorrhagic septicemia virus genotype IVb depends on exposure route. Dis Aquat Org 115:25-36. https://doi.org/10.3354/dao02872

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