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DAO 115:263-268 (2015)  -  DOI: https://doi.org/10.3354/dao02894

NOTE
New yellow head virus genotype (YHV7) in giant tiger shrimp Penaeus monodon indigenous to northern Australia

Peter G. Mohr1,*, Nicholas J. G. Moody1, John Hoad1, Lynette M. Williams1, Rachel O. Bowater2, David M. Cummins1, Jeff A. Cowley3, Mark StJ. Crane1

1CSIRO Australian Animal Health Laboratory, Geelong, VIC 3220, Australia
2Department of Agriculture, Fisheries and Forestry, Biosecurity Queensland, Townsville, QLD 4810, Australia
3CSIRO Agriculture Flagship, Queensland Bioscience Precinct, St. Lucia, QLD 4067, Australia
*Corresponding author:

ABSTRACT: In 2012, giant tiger shrimp Penaeus monodon originally sourced from Joseph Bonaparte Gulf in northern Australia were examined in an attempt to identify the cause of elevated mortalities among broodstock at a Queensland hatchery. Nucleic acid extracted from ethanol-fixed gills of 3 individual shrimp tested positive using the OIE YHV Protocol 2 RT-PCR designed to differentiate yellow head virus (YHV1) from gill-associated virus (GAV, synonymous with YHV2) and the OIE YHV Protocol 3 RT-nested PCR designed for consensus detection of YHV genotypes. Sequence analysis of the 794 bp (Protocol 2) and 359 bp (Protocol 3) amplicons from 2 distinct regions of ORF1b showed that the yellow-head-complex virus detected was novel when compared with Genotypes 1 to 6. Nucleotide identity on the Protocol 2 and Protocol 3 ORF1b sequences was highest with the highly pathogenic YHV1 genotype (81 and 87%, respectively) that emerged in P. monodon in Thailand and lower with GAV (78 and 82%, respectively) that is enzootic to P. monodon inhabiting eastern Australia. Comparison of a longer (725 bp) ORF1b sequence, spanning the Protocol 3 region and amplified using a modified YH30/31 RT-nPCR, provided further phylogenetic evidence for the virus being distinct from the 6 described YHV genotypes. The virus represents a unique seventh YHV genotype (YHV7). Despite the mortalities observed, the role of YHV7 remains unknown.


KEY WORDS: Yellow head virus · Genotype 7 · Penaeus monodon · Australia


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Cite this article as: Mohr PG, Moody NJG, Hoad J, Williams LM and others (2015) New yellow head virus genotype (YHV7) in giant tiger shrimp Penaeus monodon indigenous to northern Australia. Dis Aquat Org 115:263-268. https://doi.org/10.3354/dao02894

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