DAO 116:191-197 (2015)  -  DOI: https://doi.org/10.3354/dao02924

Immunohistochemical characterization of intestinal neoplasia in zebrafish Danio rerio indicates epithelial origin

Colleen E. Paquette1,4, Michael L. Kent 1, Tracy S. Peterson1,5, Rong Wang2, Roderick H. Dashwood2,6, Christiane V. Löhr3,*

1Department of Microbiology, Oregon State University, Corvallis, Oregon, USA
2Linus Pauling Institute, Oregon State University, Corvallis, Oregon, USA
3Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, Oregon, USA
4Present address: Translational Imaging Center, University of Southern California, Los Angeles, California, USA
5Present address: University of Arkansas Regulatory Science Center, Pine Bluff, Arkansas, USA
6Present address: Center for Epigenetics & Disease Prevention, Texas A&M Health Science Center, Houston, Texas, USA
*Corresponding author:

ABSTRACT: Spontaneous neoplasia of the intestinal tract in sentinel and moribund zebrafish Danio rerio is common in some zebrafish facilities. We previously classified these tumors as adenocarcinoma, small-cell carcinoma, or carcinoma otherwise unspecified based on histomorphologic characteristics. Based on histological presentation, the primary differential diagnosis for the intestinal carcinomas was tumor of neuroendocrine cells (e.g. carcinoids). To further characterize the phenotype of the neoplastic cells, select tissue sections were stained with a panel of antibodies directed toward human epithelial (cytokeratin wide spectrum screening [WSS], AE1/AE3) or neuroendocrine (S100, chromogranin A) markers. We also investigated antibody specificity by Western blot analysis, using a human cell line and zebrafish tissues. Nine of the intestinal neoplasms (64%) stained for AE1/AE3; 7 (50%) also stained for WSS. None of the intestinal neoplastic cells stained for chromogranin A or S100. Endocrine cells of the pituitary gland and neurons and axons of peripheral nerves and ganglia stained for chromogranin A, whereas perineural and periaxonal cells of peripheral intestinal ganglia, and glial and ependymal cells of the brain stained for S100. Immunohistochemistry for cytokeratins confirmed the majority of intestinal neoplasms in this cohort of zebrafish as carcinomas.


KEY WORDS: Zebrafish · Neoplasia · Intestine · Carcinoma · Immunohistochemistry · Cytokeratin · Western blot


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Cite this article as: Paquette CE, Kent ML, Peterson TS, Wang R, Dashwood RH, Löhr CV (2015) Immunohistochemical characterization of intestinal neoplasia in zebrafish Danio rerio indicates epithelial origin. Dis Aquat Org 116:191-197. https://doi.org/10.3354/dao02924

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