DAO 117:13-20 (2015)  -  DOI: https://doi.org/10.3354/dao02925

Cynatratoside-C efficacy against theronts of Ichthyophthirius multifiliis, and toxicity tests on grass carp and mammal blood cells

Yao-Wu Fu1, Qi-Zhong Zhang1,*, De-Hai Xu2, Bin Wang1, Jing-Han Liang1, De-Jie Lin1

1Institute of Hydrobiology, Jinan University, Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering Ministry of Education, Guangzhou 510632, PR China
2United States Department of Agriculture, Agricultural Research Service, Aquatic Animal Health Research Unit, 990 Wire Road, Auburn, AL 36832-4352, USA
*Corresponding author:

ABSTRACT: Infection by Ichthyophthirius multifiliis, a ciliated protozoan parasite, results in high fish mortality and causes severe economic losses in aquaculture. To find new, efficient anti-I. multifiliis agents, cynatratoside-C was isolated from Cynanchum atratum by bioassay-guided fractionation in a previous study. The present study investigated the anti-theront activity, determined the toxicity of cynatratoside-C to grass carp Ctenopharyngodon idellus and mammalian blood cells, and evaluated the protection of cynatratoside-C against I. multifiliis theront infection in grass carp. Results showed that all theronts were killed by 0.25 mg l-1 of cynatratoside-C in 186.7 ± 5.8 min. Cynatratoside-C at 0.25 mg l-1 was effective in treating infected grass carp and protecting naive fish from I. multifiliis infestation. The 96 h median lethal concentration (LC50) of cynatratoside-C to grass carp and 4 h median effective concentration (EC50) of cynatratoside-C to theront were 46.8 and 0.088 mg l-1, respectively. In addition, the hemolysis assay demonstrated that cynatratoside-C had no cytotoxicity to rabbit red blood cells. Therefore, cynatratoside-C could be a safe and effective potential parasiticide for controlling I. multifiliis.


KEY WORDS: Cynatratoside-C · Cynanchum atratum · Ctenopharyngodon idellus · Antiparasitic activity · Toxicity · Ichthyophthirius multifiliis


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Cite this article as: Fu YW, Zhang QZ, Xu DH, Wang B, Liang JH, Lin DJ (2015) Cynatratoside-C efficacy against theronts of Ichthyophthirius multifiliis, and toxicity tests on grass carp and mammal blood cells. Dis Aquat Org 117:13-20. https://doi.org/10.3354/dao02925

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