DAO 117:177-185 (2016)  -  DOI: https://doi.org/10.3354/dao02950

Germ-free sea bass Dicentrarchus labrax larval model: a valuable tool in the study of host-microbe interactions

M. Schaeck1,*, E. De Swaef1, W. Van den Broeck1, S. Van Nevel2, N. Boon2, N. De Geyter3, R. Morent3, K. Demeestere4, L. Duchateau5, C. Coulombet6, F. Haesebrouck7, A. Decostere

1Department of Morphology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium
2Laboratory of Microbial Ecology and Technology (LabMET), Faculty of Bioscience Engineering, Ghent University, Coupure links 653, 9000 Gent, Belgium
3Department of Applied Physics, Faculty of Engineering and Architecture, Ghent University, Sint-Pietersnieuwstraat 41 B4, 9000 Gent, Belgium
4Research Group EnVOC, Department of Sustainable Organic Chemistry and Technology, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, 9000 Gent, Belgium
5Department of Comparative Physiology and Biometry, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium
6Ecloserie Marine de Gravelines, Gravelines, France
7Department of Pathology, Bacteriology and Poultry diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium
*Corresponding author:

ABSTRACT: A thorough understanding of host-microbe interactions is crucial for more efficient disease management in the marine larviculture industry. As demonstrated in terrestrial animal research, gnotobiotic systems (involving animals cultured in germ-free conditions or inoculated with known microorganisms) are excellent tools to extend our understanding of the mechanisms involved in host-microbe interactions and allow the evaluation of new treatments for diseases. In this study, we introduce a germ-free European sea bass Dicentrarchus labrax larval model, independent of the continuous addition of antimicrobial agents. This model has an experimental set-up that allows addition of live feed to the larvae without compromising the germ-free status. This model will facilitate and render aquaculture research more effective in terms of mitigation fish larval diseases.


KEY WORDS: Aquaculture · Gnotobiotic · Pathogen · Larvae


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Cite this article as: Schaeck M, De Swaef E, Van den Broeck W, Van Nevel S and others (2016) Germ-free sea bass Dicentrarchus labrax larval model: a valuable tool in the study of host-microbe interactions. Dis Aquat Org 117:177-185. https://doi.org/10.3354/dao02950

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