DAO 118:45-53 (2016)  -  DOI: https://doi.org/10.3354/dao02955

Prevalence of the pathogen Aphanomyces astaci in freshwater crayfish populations in Croatia

Ivana Maguire1,*, Mišel Jelić1, Goran Klobučar1, Mylène Delpy2, Carine Delaunay2, Frederic Grandjean2

1University of Zagreb, Faculty of Science, Department of Biology, Rooseveltov trg 6, 10000 Zagreb, Croatia
2Universite de Poitiers, Laboratoire ‘Ecologie et Biologie des Interactions’, Equipe ‘Ecologie, Evolution, Symbiose’, UMR CNRS 7267, 6 rue Michel Brunet, Bat B 35, 86022 Poitiers Cedex, France
*Corresponding author:

ABSTRACT: The Oomycete Aphanomyces astaci is an obligate crayfish parasite that co-evolved with American crayfish species, and they therefore generally live in a balanced relationship. On the contrary, European native crayfish are highly susceptible to A. astaci, and infestation with it causes development of the lethal disease termed crayfish plague. Until now, 5 A. astaci strains have been described from the freshwater crayfish present in Europe. In this study we aimed to investigate the occurrence of the pathogen A. astaci in Croatian native and non-native crayfish populations, as well as to genotype established strains using microsatellite markers and obtain information on the pathogen’s epidemiology. Our results showed that the pathogen is widespread in both native and non-native crayfish populations. Agent level, when positive, in non-native crayfish was generally low; in native species it was higher. Genotyping from microsatellites proved the presence of the B (Ps) strain in non-native species (Pacifastacus leniusculus), while the A (As) strain was detected from viable native species (Astacus astacus and Austropotamobius torrentium) that are distributed in areas lacking non-native crayfish. The genotype from A. torrentium differed from a typical A (As) by 1 allele. Strain B (Ps) was identified in native Astacus leptodactylus from the population that co-occurs with P. leniuscuslus. Interestingly, in 1 A. leptodactylus population both A (As) and B (Ps) strains were present.


KEY WORDS: Crayfish plague · Non-native crayfish · Native crayfish · Genotyping


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Cite this article as: Maguire I, Jelić M, Klobučar G, Delpy M, Delaunay C, Grandjean F (2016) Prevalence of the pathogen Aphanomyces astaci in freshwater crayfish populations in Croatia. Dis Aquat Org 118:45-53. https://doi.org/10.3354/dao02955

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