DAO 119:173-177 (2016)  -  DOI: https://doi.org/10.3354/dao02987

NOTE
ORF13 in the Type III secretion system gene cluster of Edwardsiella tarda binds to the mammalian factor Cugbp2

Jun Okuda1,2,*, Yusuke Takeuchi1, Masashi Yasuda2, Toshihiro Nakai1

1Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan
2Department of Microbiology, Kagawa Prefectural University of Health Sciences 281-1 Hara, Mure-cho, Takamatsu, Kagawa 761-0123, Japan
*Corresponding author:

ABSTRACT: The Type III secretion system (TTSS) is essential for the intracellular replication of Edwardsiella tarda in phagocytes of fish and mammals, and a hypothetical gene (orf13) located in the TTSS gene cluster is required for intracellular replication and virulence of E. tarda. Here, we show that under TTSS-inducing conditions, the protein ORF13 was secreted into culture supernatant. Then, using a yeast 2-hybrid screen, we show that the mammalian factor Cugbp2, which regulates apoptosis in breast cancer cells, directly interacts with ORF13. A pull-down assay revealed that ORF13 binds to the C-terminal region of Cugbp2. Our results suggest that ORF13 may facilitate E. tarda replication in phagocytes by binding to Cugbp2.


KEY WORDS: Edwardsiella tarda · Type III secretion system · ORF13 · Mammalian factor Cugbp2


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Cite this article as: Okuda J, Takeuchi Y, Yasuda M, Nakai T (2016) ORF13 in the Type III secretion system gene cluster of Edwardsiella tarda binds to the mammalian factor Cugbp2. Dis Aquat Org 119:173-177. https://doi.org/10.3354/dao02987

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