DAO 119:199-206 (2016)  -  DOI: https://doi.org/10.3354/dao03000

Metabolic effects of parasitization by the barnacle Polyascus plana (Cirripedia: Rhizocephala: Sacculinidae) on a grapsid host, Metopograpsus thukuhar

Chia-Jen Hsiao1,*, Yen-I Wu2,*, Tzu-An Tung2, Guan-Yi Wang2, Jean-Yves Toullec3,4, Shih-Ting Liu2, Wen-San Huang5,6, Chi-Ying Lee2,**

1Division of Gastroenterology, Department of Internal Medicine, Taoyuan Armed Forces General Hospital, Taoyuan 32551, Taiwan
2Department of Biology, National Changhua University of Education, Changhua 50058, Taiwan
3Sorbonne Universités, UPMC Université Paris 06, UMR 7144 CNRS, Equipe ABICE, Station Biologique de Roscoff, Roscoff, France
4CNRS, UMR 7144, Adaptation et Diversité en Milieu Marin, Station Biologique de Roscoff, Roscoff, France
5Department of Biology, National Museum of Natural Science, Taichung 40453, Taiwan
6Department of Life Sciences, National Chung Hsing University, Taichung 40254, Taiwan
*These 2 authors contributed equally to this work
**Corresponding author:

ABSTRACT: Pathophysiological studies of rhizocephalan infections are rare. We describe differences in the levels of tissue and hemolymph metabolites between Polyascus plana-parasitized and unparasitized individuals of Metopograpsus thukuhar. Crabs were assigned to either a parasitized (carrying at least 1 externa, i.e. a protruding reproductive body) or an unparasitized (not carrying externae and determined to be rootlet-free by a barnacle 18S rRNA-based polymerase chain reaction) group. Quantification of metabolites showed that muscle glycogen levels were significantly lower and hepatopancreas levels were significantly higher in parasitized crabs compared to unparasitized crabs; hepatopancreas triacylglycerol levels were significantly higher and hemolymph levels significantly lower in parasitized hosts, and there was no significant difference in muscle triacylglycerol levels between unparasitized and parasitized animals. Glucose levels in the hepatopancreas, muscle, and hemolymph were all significantly higher in parasitized hosts. Significant levels of glucose, triacylglycerol, and glycogen were present in the barnacle externae. In addition, levels of crustacean hyperglycemic hormone in the sinus glands were not significantly different between unparasitized and parasitized animals. Glucose mobilized from the muscle is likely converted to glycogen and triacylglycerol in the rootlet-infiltrated hepatopancreas of parasitized hosts, and the eyestalk neuroendocrine system appears not to be significantly impaired, in terms of hormone production and storage, by parasitization.


KEY WORDS: Parasitism · Rhizocephala · Metabolism · Crustacean hyperglycemic hormone


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Cite this article as: Hsiao CJ, Wu YI, Tung TA, Wang GY and others (2016) Metabolic effects of parasitization by the barnacle Polyascus plana (Cirripedia: Rhizocephala: Sacculinidae) on a grapsid host, Metopograpsus thukuhar. Dis Aquat Org 119:199-206. https://doi.org/10.3354/dao03000

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