DAO 122:105-123 (2016)  -  DOI: https://doi.org/10.3354/dao03068

Detection of infectious spleen and kidney necrosis virus (ISKNV) and turbot reddish body iridovirus (TRBIV) from archival ornamental fish samples

J. Go1,8, T. B. Waltzek2, K. Subramaniam2, S. C. Yun3, J. M. Groff4, I. G. Anderson5, R. Chong5, I. Shirley6, J. C. L. Schuh6,9, J. H. Handlinger7, A. Tweedie1, R. J. Whittington1,*

1Faculty of Veterinary Science, University of Sydney, NSW 2570, Australia
2Department of Infectious Diseases and Pathology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32608, USA
3Department of Medicine and Epidemiology, Veterinary Teaching Hospital, School of Veterinary Medicine, University of California, Davis, CA 95616, USA
4Department of Pathology, Veterinary Teaching Hospital, School of Veterinary Medicine, University of California, Davis, CA 95616, USA
5Queensland Department of Agriculture, Fisheries and Forestry (QDAFF), Biosecurity Sciences Laboratory, Health and Food Sciences Precinct, Coopers Plains, QLD 4108, Australia
6Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 0W0, Canada
7DPIPWE Animal Health Laboratory, 165 Westbury Rd, Prospect, TAS 7250, Australia
8Present address: NSW Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Menangle, NSW 2568, Australia
9Present address: JCL Schuh, PLLC, Bainbridge Island, WA 98110-3663, USA
*Corresponding author:

ABSTRACT: Although infections caused by megalocytiviruses have been reported from a wide range of finfish species for several decades, molecular characterisation of the viruses involved has been undertaken only on more recent cases. Sequence analysis of the major capsid protein and adenosine triphosphatase genes is reported here from formalin-fixed, paraffin-embedded material from 2 archival ornamental fish cases from 1986 and 1988 in conjunction with data for a range of genes from fresh frozen tissues from 5 cases obtained from 1991 through to 2010. Turbot reddish body iridovirus (TRBIV) genotype megalocytiviruses, previously not documented in ornamental fish, were detected in samples from 1986, 1988 and 1991. In contrast, megalocytiviruses from 1996 onwards, including those characterised from 2002, 2006 and 2010 in this study, were almost indistinguishable from infectious spleen and kidney necrosis virus (ISKNV). Three of the species infected with TRBIV-like megalocytiviruses from 1986 to 1991, viz. dwarf gourami Trichogaster lalius (formerly Colisa lalia), freshwater angelfish Pterophyllum scalare and oscar Astronotus ocellatus, were infected with ISKNV genotype megalocytiviruses from 2002 to 2010. The detection of a TRBIV genotype isolate in ornamental fish from 1986 represents the index case, confirmed by molecular sequence data, for the genus Megalocytivirus.


KEY WORDS: Megalocytivirus · Iridovirus · Phylogenetics · Molecular epidemiology · Ornamental fish · ISKNV · TRBIV · Red seabream iridovirus · RSIV · Taxonomy


Full text in pdf format
Supplementary material 
Cite this article as: Go J, Waltzek TB, Subramaniam K, Yun SC and others (2016) Detection of infectious spleen and kidney necrosis virus (ISKNV) and turbot reddish body iridovirus (TRBIV) from archival ornamental fish samples. Dis Aquat Org 122:105-123. https://doi.org/10.3354/dao03068

Export citation
Mail this link - Contents Mailing Lists - RSS
- -