DAO 125:19-29 (2017)  -  DOI: https://doi.org/10.3354/dao03129

Treatment of Francisella infections via PLGA- and lipid-based nanoparticle delivery of antibiotics in a zebrafish model

Lilia S. Ulanova1,*, Marina Pinheiro2, Carina Vibe1, Claudia Nunes2, Dorna Misaghian3, Steven Wilson3, Kaizheng Zhu3, Federico Fenaroli1, Hanne C. Winther-Larsen4,5, Salette Reis2, Gareth Griffiths

1Department of Biosciences, University of Oslo, 0371 Oslo, Norway
2Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal
3Department of Chemistry, University of Oslo, 0315 Oslo, Norway
4School of Pharmacy and 5Center for Integrative Microbial Evolution, University of Oslo, 0371 Oslo, Norway
*Corresponding author:

ABSTRACT: We tested the efficiency of 2 different antibiotics, rifampicin and oxolinic acid, against an established infection caused by fish pathogen Francisella noatunensis ssp. orientalis (F.n.o.) in zebrafish. The drugs were tested in the free form as well as encapsulated into biodegradable nanoparticles, either polylactic-co-glycolic acid (PLGA) nanoparticles or nanostructured lipid carriers. The most promising therapies were PLGA-rifampicin nanoparticles and free oxolinic acid; the PLGA nanoparticles significantly delayed embryo mortality while free oxolinic acid prevented it. Encapsulation of rifampicin in both PLGA and nanostructured lipid carriers enhanced its efficiency against F.n.o. infection relative to the free drug. We propose that the zebrafish model is a robust, rapid system for initial testing of different treatments of bacterial diseases important for aquaculture.


KEY WORDS: Nanoparticles · Francisella orientalis · Infection · Zebrafish embryo · Antibiotics · Danio rerio


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Cite this article as: Ulanova LS, Pinheiro M, Vibe C, Nunes C and others (2017) Treatment of Francisella infections via PLGA- and lipid-based nanoparticle delivery of antibiotics in a zebrafish model. Dis Aquat Org 125:19-29. https://doi.org/10.3354/dao03129

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