DAO 32:237-239 (1998)  -  doi:10.3354/dao032237

Differentiation of BP-type baculovirus strains using in situ hybridization

S. Durand1,*, D. V. Lightner2, J. R. Bonami1

1UMR 219 CNRS/IFREMER, UMII, Place E. bataillon, F-34095 Montpellier Cedex 05, France
2Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona 85721, USA

BP-type viruses infect wild and farmed shrimp species in the Americas and can cause high mortality in larval stages. Histologically, BP disease is characterized by the presence of occlusion bodies (OBs) in the nucleus of hepatopancreatic epithelial cells. By in situ hybridization using different molecular probes, we tested shrimp infected with BP-type viruses collected from several geographical areas. In one shrimp, a case of double infection by 2 different BP-type viruses was noted. This has not been previously reported. The molecular probes recognized only the Pacific strain of BP. This specificity of the probe was confirmed by in situ hybridization tests with some infected shrimp collected from the Atlantic and Pacific coasts. The probes reacted only with infected shrimp obtained from the Pacific coast. These results suggest the existence of at least 2 different BP-type viruses and show that specific probes can be used to differentiate between them.

Baculovirus · BP · In situ hybridization · Non- radioactive probes · Shrimp

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