DAO 33:73-75 (1998)  -  doi:10.3354/dao033073

Generation and preliminary characterisation of monoclonal antibodies directed to glycerophospholipid:cholesterol acyltransferase (GCAT) native epitopes of Aeromonas salmonicida

Ingolf Lachmann*, Ulrich Wagner, Dietlind Hädge, Karl Drössler

Institute of Zoology, University of Leipzig, Talstrasse 33, D-04103 Leipzig, Germany

Four monoclonal antibodies (MAbs) directed to native glycerophospholipid:cholesterol acyltransferase (GCAT) epitopes of Aeromonas salmonicida were isolated using an esterase capture assay. The molecular mass of this MAb-defined antigen was estimated to be 26 kDa in SDS-PAGE. Three different epitope specificities of these MAbs were demonstrated. It was shown that all 4 MAbs recognize GCAT in culture filtrates of the strain MT004 excluding the simultaneous trapping of other components. None of the MAbs react with the denatured GCAT in Western blots.


Aeromonas salmonicida · GCAT · Monoclonal antibodies


Full text in pdf format