DAO 34:231-237 (1998)  -  doi:10.3354/dao034231

Cloning and expression of a putative glycoprotein gene of channel catfish virus using baculovirus expression system

Huseyin Kucuktas1, Yolanda J. Brady1,*, Sadik Tuzun2

1Department of Fisheries and Allied Aquacultures and 2Department of Plant Pathology, Auburn University, Auburn, Alabama 36849, USA
*Addressee for correspondence. E-mail: ybrady@acesag.auburn.edu

ABSTRACT: The 1035 base-pair (bp) open reading frame 59 (ORF59) of channel catfish virus (CCV) codes for an abundant hydrophobic membrane glycoprotein considered to be the major envelope glycoprotein of the virus. The ORF59 gene of CCV was cloned into the baculovirus genome by site-specific transposition and expressed in Spodoptera frugiperda cells (Sf9 cells). Expressed protein could be recognized by polyclonal antibody against CCV and appeared to have the same electrophoretic mobility as native protein. Crude lysates of Sf9 cells infected with recombinant baculovirus containing ORF59 gene inhibited plaque formation of CCV on channel catfish ovary cells.

KEY WORDS: Channel catfish virus · Glycoprotein · Baculovirus expression

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