DAO 42:233-236 (2000)  -  doi:10.3354/dao042233

Lipid peroxidation induced by Clinostomum detruncatum in muscle of the freshwater fish Rhamdia quelen

A. R. R. Belló1, E. Fortes1, A. Belló-Klein2,*, A. A. Belló2, S. F. Llesuy2, R. B. Robaldo3, A. Bianchini3

1Departamento de Parasitologia and
2Departamento de Fisiologia, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, 500, 90050-170, Porto Alegre-RS, Brazil
3Departamento de Ciências Fisiológicas, Fundação Universidade Federal do Rio Grande, R. Eng. Alfredo Huch, 475, 96201-900, Rio Grande-RS, Brazil
*Corresponding author. E-mail:

ABSTRACT: The effect of Clinostomum detruncatum metacercaria infection on the activities of the antioxidant enzymes superoxide dismutase and catalase in muscle of the freshwater fish Rhamdia quelen was analyzed. Tert-butyl hydroperoxide-initiated chemiluminescence, a measure of lipid peroxidation, was also investigated. Enzyme activities were similar in infected and uninfected fishes. However, the chemiluminescence was almost 2-fold higher in muscle of infected fishes than in muscle of uninfected ones. These results indicate that parasite infection induces oxidative stress and a higher level of membrane damage in the fish muscle due to an imbalance between pro-oxidants and non-enzymatic antioxidants. Our results suggest that fish response to parasite infection could involve, as in other vertebrates, reactive oxygen intermediates.

KEY WORDS: Parasite · Oxidative stress · Lipid peroxidation · Fish

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