DAO 45:115-120 (2001)  -  doi:10.3354/dao045115

Effect of lectins on the invasion of Ichthyophthirius theront to channel catfish tissue

De-Hai Xu*, Phillip H. Klesius, Craig A. Shoemaker

United States Department of Agriculture, Agricultural Research Service, Aquatic Animal Health Research Laboratory, 990 Wire Road, Auburn, Alabama 36831, USA

ABSTRACT: This study determined the effects of lectin binding to theronts of Ichthyophthirius multifiliis on theront immobilization, invasion, trophont development and survival in channel catfish Ictalurus punctatus excised fins in vitro. Soybean agglutinin (SBA), lentil agglutinin (LCA), gorse agglutinin (UEA-I) and wheat germ agglutinin (WGA) were used to treat theronts. Percentages of theronts immobilized by 4 lectins ranged from 12.0 to 19.4% at a concentration of 1000 µg ml-1. These lectins bound more than half of the theronts at a concentration of 50 µg ml-1. More theronts were labeled by SBA and WGA than by lectin LCA at concentrations of 50 and 100 µg ml-1, respectively. The binding of these lectins to theronts indicated that monosaccharides (d-galactose, l-fucose, d mannose and d-glucose) and amino sugar derivatives (N-acetylgalactosamine and N-acetylglucosamine) were present on the surface of theronts. Invasion was reduced significantly for theronts treated with LCA, UEA-I and WGA. No difference in invasion was found between control and SBA bound theronts (p > 0.05). The binding of lectin LCA, UEA-I and WGA to theronts significantly reduced the development of trophonts (p < 0.05). The mean volumes of trophonts labeled with these 3 lectins were smaller than volumes in control trophonts from 8 to 48 h after exposure. Survival was lower in trophonts labeled with lectins than in control trophonts at 48 h after exposure.


KEY WORDS: Lectins · Ichthyophthirius · Immobilization · Invasion · Development · Tissue


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