DAO 48:143-148 (2002)  -  doi:10.3354/dao048143

Genetic relatedness among Japanese, American and European isolates of viral hemorrhagic septicemia virus (VHSV) based on partial G and P genes

T. Nishizawa1,*, H. Iida1, R. Takano1, T. Isshiki2, K. Nakajima3, K. Muroga1

1Faculty of Applied Biological Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan
2Kagawa Prefectural Fisheries Experiment Station, Takamatsu 761-0111, Japan
3National Research Institute of Aquaculture, Nansei, Mie 516-0193, Japan

ABSTRACT: Molecular virological analyses of 8 Japanese VHSV (viral hemorrhagic septicemia virus) isolates from wild and farmed Japanese flounder Paralichthys olivaceus were performed to investigate their genetic relatedness to American and European isolates of VHSV. Phylogenetic analyses based on the partial nucleotide sequences of G and P genes revealed that there are 2 genogroups of VHSV in Japan. The first one represented by the Obama25 isolate is closely related to the American isolates (Genogroup I) while the other, the KRRV9601 isolate, is closely related to the traditional European isolates (Genogroup III). The 2 types of Japanese VHSV showed differences in the relative mobility of the G protein and intensity of the antibody reaction on the P and M proteins. The Obama25 type of VHSV is widely distributed as a native virus in the coastal areas of western Japan and has been responsible for the occurrence of VHSV infection in farmed Japanese flounder while the KRRV9601 isolate is considered to have been introduced from a foreign country.


KEY WORDS: Viral hemorrhagic septicemia virus · Paralichthys olivaceus · VHSV · phylogenetic analysis · G gene · P gene · M1 gene


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