Detection of the parasitic dinoflagellate Hematodinium in the  Norway lobster Nephrops norvegicus by ELISA

H. J. Small; S. Wilson; D. M. Neil; P. Hagan; G. H. Coombs

doi:10.3354/dao052175

DAO

Diseases of Aquatic Organisms

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DAO 52:175-177 (2002) - doi:10.3354/dao052175

Detection of the parasitic dinoflagellate Hematodinium in the Norway lobster Nephrops norvegicus by ELISA

H. J. Small^1,*, S. Wilson², D. M. Neil¹, P. Hagan², G. H. Coombs²

¹Division of Environmental and Evolutionary Biology, Graham Kerr Building, and
²Division of Infection and Immunity, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, Scotland, UK

*E-mail: 9909258s@student.gla.ac.uk

ABSTRACT: Norway lobsters Nephrops norvegicus from the coastal waters of Scotland are seasonally infected by a parasitic dinoflagellate of the genus Hematodinium. An enzyme-linked immunosorbent assay (ELISA) has been developed for the detection of the parasite in the haemolymph of N. norvegicus. The ELISA is simple to perform with a detection limit of 5 x 10⁴ parasites ml^-1 haemolymph. The ELISA is currently being used to study the prevalence and seasonality of Hematodinium infection in N. norvegicus and other crustacean hosts.

KEY WORDS: Hematodinium · Nephrops norvegicus · Infection · ELISA

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DAO 52:175-177 (2002) - doi:10.3354/dao052175

Detection of the parasitic dinoflagellate Hematodinium in the Norway lobster Nephrops norvegicus by ELISA

H. J. Small1,*, S. Wilson2, D. M. Neil1, P. Hagan2, G. H. Coombs2

H. J. Small^1,*, S. Wilson², D. M. Neil¹, P. Hagan², G. H. Coombs²