DAO 62:147-153 (2004)  -  doi:10.3354/dao062147

Effect of polychlorinated biphenyls (Delor 103) on haematological and enzyme parameters of the rainbow trout Oncorhynchus mykiss

J. Rehulka1,*, B. Minarík2

1Department of Zoology, Silesian Museum, Tyrsova 1, 746 46 Opava, Czech Republic
2Department of Statistics, Faculty of Economics, Mendel University of Agriculture and Forestry, 613 00 Brno, Czech Republic

ABSTRACT: Rainbow trout at a weight of 223 ± 12 g (mean ± SD) were experimentally injected with a technical mixture of Delor 103 to evaluate the red blood cell indices (red blood cell count, haematocrit, haemoglobin, mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration) and some biochemical and enzyme parameters of the blood plasma (total protein, glucose, inorganic phosphate, total calcium, sodium, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, lactate dehydrogenase). Delor 103, administered by the i.p. route at a concentration of 0.24 g kg–1 120 h–1, caused an increase in the red blood cell counts, haematocrit values, haemoglobin concentrations, inorganic phosphate, alanine aminotransferase and lactate dehydrogenase. The sodium level fell. The fish injected with Delor 103 showed a relative decrease in the lymphocyte count and a relative increase in the count of neutrophile band forms.

KEY WORDS: Trichlor biphenyl · Toxicity · Red blood cell indices · Biochemical indices · Differential leucocyte

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