DAO 62:233-238 (2004)  -  doi:10.3354/dao062233

Occurrence of scuticociliatosis in olive flounder Paralichthys olivaceus by Phiasterides dicentrarchi (Ciliophora: Scuticociliatida)

Sung Mi Kim1, Jae Bum Cho1, Sung Koo Kim2, Yoon Kwon Nam3, Ki Hong Kim1,*

1Department of Aquatic Life Medicine, 2Faculty of Food Science and Biotechnology, and 3Department of Aquaculture, Pukyong National University, Pusan 608-737, Korea
*Corresponding author. Email:

ABSTRACT: In the course of identifying scuticociliates recently obtained from systemically infected olive flounder Paralichthys olivaceus in Korea, we found a scuticociliate species whose small subunit ribosomal RNA (SS rRNA) gene was not amplified by species-specific primers previously designed for Uronema marinum and Pseudocohnilembus persalinus. By studying morphological characteristics of wet-mounted and stained specimens, we identified the species as Philasterides dicentrarchi, which has been reported to cause systemic infection in the European sea bass Dicentrarchus labrax and turbot Scophthalmus maximus. In this study, we compared morphological characteristics of our specimens with previously reported Philasterides species, including P. dicentrarchi, and sequenced the SS rRNA gene in order to design P. dicentrarchi specific primers. This is the first report on scuticociliatosis caused by P. dicentrarchi from marine fish in Asia.


KEY WORDS: Philasterides dicentrarchi · Scuticociliatosis · Olive flounder · Morphology · SS rRNA gene · PCR-based


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