DAO 64:135-139 (2005)  -  doi:10.3354/dao064135

SSU rDNA analysis of Kudoa rosenbuschi (Myxosporea) from the Argentinean hakeMerluccius hubbsi

Elvira Abollo1,2,*, Beatriz Novoa1, Antonio Figueras1

1Instituto de Investigaciones Marinas (CSIC), Eduardo Cabello 6, 36208 Vigo, Spain
2Present address: Centro de Investigacions Mariñas, Conselleria de Pesca e Asuntos Maritimos, Xunta de Galicia,Apartado 13, 36620 Vilanova de Arousa, Spain

ABSTRACT: The cloning and sequencing of the small subunit (SSU) ribosomal DNA gene from Kudoa rosenbuschi (myxosporean species associated with post-mortem myoliquefaction process in the Argentinean hake Merluccius hubbsi) is reported. The SSU rDNA was found to contain 1740 bp with a single polymorphic site with either a C or T at position 221. The sequence data obtained in this study and those known sequences of Kudoa species deposited in the GenBank were all analyzed to construct a phylogenetic tree. Nucleotide sequences showed the highest degree of identity with K. funduli, followed by K. miniauriculata, K. clupeidae and K. dianae. Phylogenetic analysis placed K. rosenbuschi in the same branch of K. clupeidae and K. funduli, and showed it to be closely related to K. dianae, K. paniformis and K. miniauriculata.

KEY WORDS: Kudoa · Myxozoa · SSU rDNA · Hake · Merluccius hubbsi

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