DAO 75:217-220 (2007)  -  doi:10.3354/dao075217

ABSTRACT: Edwardsiella tarda glyceraldehyde-3-phosphate dehydrogenase (GAPDH) may be an effective vaccine candidate against infection by E. tarda in Japanese flounder Paralichthys olivaceus. The GAPDH of E. tarda is highly homologous to that of Vibrio cholerae (91%), and therefore E. tarda GAPDH may have protective antigenicity against Vibrio species. In this study, we immunized Japanese flounder with GAPDH of E. tarda and infected the fish with V. anguillarum. The result showed that GAPDH prepared from E. tarda protected Japanese flounder effectively in a challenge of V. anguillarum. Therefore, E. tarda GAPDH should be considered as a multi-purpose vaccine candidate against several kinds of pathogenic bacteria.

KEY WORDS: Glyceraldehyde-3-phosphate dehydrogenase · GAPDH · Protective antigenicity · Multi-purpose vaccine candidate · Edwardsiella tarda · Vibrio anguillarum