DAO 75:217-220 (2007)  -  doi:10.3354/dao075217

Glyceraldehyde-3-phosphate dehydrogenase of Edwardsiella tarda has protective antigenicity against Vibrio anguillarum in Japanese flounder

Ying Liu1, Syun-ichirou Oshima2,*, Kenji Kawai1

1Fish Disease Laboratory, Department of Aquaculture, Faculty of Agriculture, and 2Laboratory of Cell Structure and Function, Division of Marine Bioresource Science, Graduate School of Kuroshio Science, Kochi University, Nankoku, Kochi 783-8502, Japan
*Corresponding author. Email:

ABSTRACT: Edwardsiella tarda glyceraldehyde-3-phosphate dehydrogenase (GAPDH) may be an effective vaccine candidate against infection by E. tarda in Japanese flounder Paralichthys olivaceus. The GAPDH of E. tarda is highly homologous to that of Vibrio cholerae (91%), and therefore E. tarda GAPDH may have protective antigenicity against Vibrio species. In this study, we immunized Japanese flounder with GAPDH of E. tarda and infected the fish with V. anguillarum. The result showed that GAPDH prepared from E. tarda protected Japanese flounder effectively in a challenge of V. anguillarum. Therefore, E. tarda GAPDH should be considered as a multi-purpose vaccine candidate against several kinds of pathogenic bacteria.


KEY WORDS: Glyceraldehyde-3-phosphate dehydrogenase · GAPDH · Protective antigenicity · Multi-purpose vaccine candidate · Edwardsiella tarda · Vibrio anguillarum


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