DAO 78:23-28 (2007)  -  DOI: https://doi.org/10.3354/dao01858

Detection of Cyprinid herpesvirus-3 (CyHV-3) DNA in infected fish tissues by nested polymerase chain reaction

Mansour El-Matbouli1,*, Ute Rucker1, Hatem Soliman1,2

1Institute of Zoology, Fish Biology and Fish Diseases, Faculty of Veterinary Medicine, University of Munich, Kaulbachstr. 37, 80539 Munich, Germany
2Veterinary Serum and Vaccine Research Institute, El-Sekka El-Beda St., PO Box 131, Abbasia, Cairo, Egypt

ABSTRACT: Diagnostic tests with high analytical sensitivity are required to detect Cyprinid herpesvirus-3 (CyHV-3) in carriers that have been implicated in the dissemination of this important disease of koi and common carp Cyprinus carpio. A nested polymerase chain reaction (PCR) test was developed to detect CyHV-3 DNA in tissues of infected fish. The 2-round PCR amplified 529 and 379 bp segments of the CyHV-3 gene coding for the major capsid protein using specific external and internal primers, respectively. The PCR test did not amplify genomic DNA from either Cyprinid herpesvirus-1 (CyHV-1) or Cyprinid herpesvirus-2 (CyHV-2). The nested PCR test detected CyHV-3 DNA at a 10-fold lower concentration than a routinely used, 1-round test. Hence, the new method should provide more sensitive diagnosis of CyHV-3 infection among koi and carp populations.


KEY WORDS: Diagnosis · Cyprinid herpesvirus-3 · CyHV-3 · Nested polymerase chain reaction · Koi · Common carp


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Cite this article as: El-Matbouli M, Rucker U, Soliman H (2007) Detection of Cyprinid herpesvirus-3 (CyHV-3) DNA in infected fish tissues by nested polymerase chain reaction. Dis Aquat Org 78:23-28. https://doi.org/10.3354/dao01858

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