DAO 79:163-168 (2008)  -  DOI: https://doi.org/10.3354/dao01896

Histopathological and ultrastructural features of enlarged cells of humpback grouper Cromileptes altivelis challenged with Megalocytivirus (family Iridoviridae) after vaccination

Ketut Mahardika1, Haryanti2, Ahmad Muzaki2, Teruo Miyazaki1,*

1Graduate school of Bioresources, Mie University, Tsu, Mie 514-8507, Japan
2Gondol Research Institute for Mariculture, PO Box 140, Singaraja, Bali Island, Indonesia
*Corresponding author. Email:

ABSTRACT: The genus Megalocytivirus in the family Iridoviridae encompasses isolates of red sea bream iridovirus (RSIV) and grouper sleepy disease iridovirus (GSDIV). In the present study, humpback grouper Cromileptes altivelis juveniles were challenged with GSDIV after vaccination with a commercial RSIV vaccine. The unvaccinated group (in duplicate) showed higher mortalities (59.3 to 66.7%) than the vaccination group (0% mortalitiy, in duplicate). Surviving fish in the vaccinated group displayed masses of enlarged cells in the spleen. Electron microscopy revealed that they contained hemosiderin granules within the cytoplasm. In contrast, moribund fish from the unvaccinated group exhibited large numbers of inclusion body-bearing cells (IBCs) in the spleen, while surviving fish displayed masses of enlarged cells in which a small number of GSDIV virions were assembled.


KEY WORDS: Humpback grouper · Cromileptes altivelis · Commercial RSIV vaccine · Grouper sleepy disease iridovirus · Enlarged cells


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Cite this article as: Mahardika K, Haryanti, Muzaki A, Miyazaki T (2008) Histopathological and ultrastructural features of enlarged cells of humpback grouper Cromileptes altivelis challenged with Megalocytivirus (family Iridoviridae) after vaccination. Dis Aquat Org 79:163-168. https://doi.org/10.3354/dao01896

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