DAO 80:69-73 (2008)  -  DOI: https://doi.org/10.3354/dao01908

NOTE
Improvement of a PCR method for the detection of necrotizing hepatopancreatitis in shrimp

Linda M. Nunan*, Carlos Pantoja, Donald V. Lightner

Department of Veterinary Sciences and Microbiology, University of Arizona, Tucson, Arizona 85721, USA

ABSTRACT: Necrotizing hepatopancreatitis (NHP) is considered to be one of the most important bacterial diseases affecting penaeid shrimp culture and is caused by an unclassified Gram-negative, pleomorphic, intracellular Alphaproteobacterium. Due to the enteric nature of the bacteria, PCR is the one non-lethal method available for detection of the pathogen. Over a decade ago, a PCR protocol was developed for detection of NHP, which over the subsequent years was shown to occasionally generate false positive reactions. The University of Arizona Aquaculture Pathology Laboratory has developed a set of primers and PCR cycling parameters that have been tested on a variety of DNA templates, using 2 types of PCR reagent systems, which eliminated the generation of false positive amplicons.


KEY WORDS: PCR · Necrotizing hepatopancreatitis · NHP · Shrimp · Penaeus vannamei


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Cite this article as: Nunan LM, Pantoja C, Lightner DV (2008) Improvement of a PCR method for the detection of necrotizing hepatopancreatitis in shrimp. Dis Aquat Org 80:69-73. https://doi.org/10.3354/dao01908

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